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The cell surface CAR- or HBGA-positive cell population did not increase after two rounds of sorting and expansion or incubating cells in human AB serum, respectively. ( A ) Live cell staining of the original or sorted and expanded CAR-positive cell populations with <t>anti-CAR-FITC</t> antibody. No enrichment of CAR-positive cells was achieved. ( B ) Live cell staining of the original (unsoaked) and human AB serum-soaked BJAB cell populations with anti-B mouse Mab and AF488-labeled secondary antibody. Soaking BJAB cells in human AB serum did not increase the number of HBGA-positive cells. One representative of three independent experiments with the BJAB-LSU cell clone is shown.
Anti Cxadr Car Antibody Fitc, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti cxadr car antibody fitc/product/Sino Biological
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anti cxadr car antibody fitc - by Bioz Stars, 2026-02
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rabbit anti human car antibody  (Cell Signaling Technology Inc)
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The cell surface CAR- or HBGA-positive cell population did not increase after two rounds of sorting and expansion or incubating cells in human AB serum, respectively. ( A ) Live cell staining of the original or sorted and expanded CAR-positive cell populations with <t>anti-CAR-FITC</t> antibody. No enrichment of CAR-positive cells was achieved. ( B ) Live cell staining of the original (unsoaked) and human AB serum-soaked BJAB cell populations with anti-B mouse Mab and AF488-labeled secondary antibody. Soaking BJAB cells in human AB serum did not increase the number of HBGA-positive cells. One representative of three independent experiments with the BJAB-LSU cell clone is shown.
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resource source identifier antibodies rabbit polyclonal anti acsf3 proteintech car  (Proteintech)
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The cell surface CAR- or HBGA-positive cell population did not increase after two rounds of sorting and expansion or incubating cells in human AB serum, respectively. ( A ) Live cell staining of the original or sorted and expanded CAR-positive cell populations with <t>anti-CAR-FITC</t> antibody. No enrichment of CAR-positive cells was achieved. ( B ) Live cell staining of the original (unsoaked) and human AB serum-soaked BJAB cell populations with anti-B mouse Mab and AF488-labeled secondary antibody. Soaking BJAB cells in human AB serum did not increase the number of HBGA-positive cells. One representative of three independent experiments with the BJAB-LSU cell clone is shown.
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The cell surface CAR- or HBGA-positive cell population did not increase after two rounds of sorting and expansion or incubating cells in human AB serum, respectively. ( A ) Live cell staining of the original or sorted and expanded CAR-positive cell populations with <t>anti-CAR-FITC</t> antibody. No enrichment of CAR-positive cells was achieved. ( B ) Live cell staining of the original (unsoaked) and human AB serum-soaked BJAB cell populations with anti-B mouse Mab and AF488-labeled secondary antibody. Soaking BJAB cells in human AB serum did not increase the number of HBGA-positive cells. One representative of three independent experiments with the BJAB-LSU cell clone is shown.
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car  (Cell Signaling Technology Inc)
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The cell surface CAR- or HBGA-positive cell population did not increase after two rounds of sorting and expansion or incubating cells in human AB serum, respectively. ( A ) Live cell staining of the original or sorted and expanded CAR-positive cell populations with <t>anti-CAR-FITC</t> antibody. No enrichment of CAR-positive cells was achieved. ( B ) Live cell staining of the original (unsoaked) and human AB serum-soaked BJAB cell populations with anti-B mouse Mab and AF488-labeled secondary antibody. Soaking BJAB cells in human AB serum did not increase the number of HBGA-positive cells. One representative of three independent experiments with the BJAB-LSU cell clone is shown.
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https://www.bioz.com/result/car/product/Cell Signaling Technology Inc
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rabbit polyclonal anti-car-1 antibody  (GenScript corporation)
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The cell surface CAR- or HBGA-positive cell population did not increase after two rounds of sorting and expansion or incubating cells in human AB serum, respectively. ( A ) Live cell staining of the original or sorted and expanded CAR-positive cell populations with <t>anti-CAR-FITC</t> antibody. No enrichment of CAR-positive cells was achieved. ( B ) Live cell staining of the original (unsoaked) and human AB serum-soaked BJAB cell populations with anti-B mouse Mab and AF488-labeled secondary antibody. Soaking BJAB cells in human AB serum did not increase the number of HBGA-positive cells. One representative of three independent experiments with the BJAB-LSU cell clone is shown.
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car d3w3g rabbit mab  (Cell Signaling Technology Inc)
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am4302 car d3w3g rabbit mab cell signaling technology  (Cell Signaling Technology Inc)
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The cell surface CAR- or HBGA-positive cell population did not increase after two rounds of sorting and expansion or incubating cells in human AB serum, respectively. ( A ) Live cell staining of the original or sorted and expanded CAR-positive cell populations with anti-CAR-FITC antibody. No enrichment of CAR-positive cells was achieved. ( B ) Live cell staining of the original (unsoaked) and human AB serum-soaked BJAB cell populations with anti-B mouse Mab and AF488-labeled secondary antibody. Soaking BJAB cells in human AB serum did not increase the number of HBGA-positive cells. One representative of three independent experiments with the BJAB-LSU cell clone is shown.

Journal: Journal of Virology

Article Title: B cell lines fail to support efficient rhesus enteric calicivirus and human norovirus replication

doi: 10.1128/jvi.00143-25

Figure Lengend Snippet: The cell surface CAR- or HBGA-positive cell population did not increase after two rounds of sorting and expansion or incubating cells in human AB serum, respectively. ( A ) Live cell staining of the original or sorted and expanded CAR-positive cell populations with anti-CAR-FITC antibody. No enrichment of CAR-positive cells was achieved. ( B ) Live cell staining of the original (unsoaked) and human AB serum-soaked BJAB cell populations with anti-B mouse Mab and AF488-labeled secondary antibody. Soaking BJAB cells in human AB serum did not increase the number of HBGA-positive cells. One representative of three independent experiments with the BJAB-LSU cell clone is shown.

Article Snippet: Recombinant anti-CXADR/CAR antibody (FITC) (SinoBiological, Cat# 10799-R271-F) and anti-A (ABO1) and anti-B (ABO2) (Diagast, Cat# 70501 and 70502) mouse monoclonal antibodies (IgM) were used for CAR and type A or type B HBGA staining, respectively.

Techniques: Staining, Labeling

Cell surface expression of CAR and the type A HBGA in rBJAB-CAR+/A + cells. ( A ) Nonpermeabilized BJAB and rBJAB-CAR+/A + cells were stained by the anti-CAR antibody (RmcB) and AF-594 secondary antibody. Cell surface expression of CAR (white arrows) could be detected only in the rBJAB-CAR+/A + cultures. ( B ) Live cell staining with anti-CAR-FITC antibody and flow cytometry analysis revealed cell surface CAR on ~99% of the rBJAB-CAR+/A + cells, while only ~1% of the parental BJAB cells stained positive. ( C ) Live cell staining and flow cytometry analysis of the rBJAB-CAR+/A + cell population with anti-A mouse Mab- and AF488-labeled secondary antibody revealed the presence of cell surface type A HBGA in ~65% of the cells. One of three individual experiments with BJAB-LSU is shown. (D) BJAB and rBJAB CAR +A + cells were infected with ReCV-FT285 (1 MOI), and infectious virus titers were evaluated by titration on LLC-MK2 monolayers at 0 hpi and 72 hpi. The mean and SD of three independent experiments are shown.

Journal: Journal of Virology

Article Title: B cell lines fail to support efficient rhesus enteric calicivirus and human norovirus replication

doi: 10.1128/jvi.00143-25

Figure Lengend Snippet: Cell surface expression of CAR and the type A HBGA in rBJAB-CAR+/A + cells. ( A ) Nonpermeabilized BJAB and rBJAB-CAR+/A + cells were stained by the anti-CAR antibody (RmcB) and AF-594 secondary antibody. Cell surface expression of CAR (white arrows) could be detected only in the rBJAB-CAR+/A + cultures. ( B ) Live cell staining with anti-CAR-FITC antibody and flow cytometry analysis revealed cell surface CAR on ~99% of the rBJAB-CAR+/A + cells, while only ~1% of the parental BJAB cells stained positive. ( C ) Live cell staining and flow cytometry analysis of the rBJAB-CAR+/A + cell population with anti-A mouse Mab- and AF488-labeled secondary antibody revealed the presence of cell surface type A HBGA in ~65% of the cells. One of three individual experiments with BJAB-LSU is shown. (D) BJAB and rBJAB CAR +A + cells were infected with ReCV-FT285 (1 MOI), and infectious virus titers were evaluated by titration on LLC-MK2 monolayers at 0 hpi and 72 hpi. The mean and SD of three independent experiments are shown.

Article Snippet: Recombinant anti-CXADR/CAR antibody (FITC) (SinoBiological, Cat# 10799-R271-F) and anti-A (ABO1) and anti-B (ABO2) (Diagast, Cat# 70501 and 70502) mouse monoclonal antibodies (IgM) were used for CAR and type A or type B HBGA staining, respectively.

Techniques: Expressing, Staining, Flow Cytometry, Labeling, Infection, Virus, Titration

Reagents and tools table

Journal: Molecular Systems Biology

Article Title: Proteome-wide copy-number estimation from transcriptomics

doi: 10.1038/s44320-024-00064-3

Figure Lengend Snippet: Reagents and tools table

Article Snippet: CAR (D3W3G) Rabbit mAb , Cell Signaling Technology , Cat # 16984.

Techniques: Recombinant, Luciferase, Sequencing, Cloning, Software, Bicinchoninic Acid Protein Assay